A new simple but effective method for breaking pollen wall by quenching into liquid nitrogen is proposed in the paper. As the result of microscope observation, breaking ratio calculation and phenol/sulphuric reaction, the maximum yield of total sugar is up to 59.588 milligram per gram of pollen(based on glucose) under the following conditions: packaged with sheet copper; defrozen at 95; frozen and defrozen for 3 times, which increases by 31.25 percent over the unprocessed one, with a high breaking ratio of 94.97%.