In order to examine possible relations between freezing properties and the number of hydroxyl groups in solute molecules,a differential scanning calorimeter(pyrisdiamond DSC) was employed to study the thermal behaviors of polyalcohols aqueous,such as hydration properties,glass transition and devitrification.Experimental results show that at low concentrations hydration behaviors have no disciplinarians.However,at high concentrations,they accrete as the concentrations augment.The different properties of cryoprotectants shown in hydration exhibit important effects on aqueous properties behaved by molecular structure,such as methyl groups and hydroxyl groups.The distinct otherness in glass transition and devitrification temperature of five polyalcohols reflects the feebleness and power in the aspects of glass transition ability.