糖的浓度和种类对人血小板冷冻干燥保存的影响
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TS205.7

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卫生部科研项目;上海市高校选拔培养优秀青年教师科研项目;上海市曙光计划科学研究基金


Influence of saccharides and their concentrations on freeze-drying of human platelets
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    摘要:

    以海藻糖、蔗糖、麦芽糖、乳糖、葡萄糖作为冻干血小板的保护剂,研究糖的浓度和种类对血小板冻干保存的影响.结果表明,冻干血小板的保存效果随海藻糖浓度的增加而提高,当海藻糖质量分数增加到20%时,非活化血小板的恢复率达到85.7%;对于质量分数均为20%不同种类的糖保护剂,非活化血小板的恢复率都达到70%以上,海藻糖略优于其他几种糖,但它们之间没有显著性差异.

    Abstract:

    Trehalose,sucrose,maltose,lactose and glucose were used as protectants for platelet freeze-drying,the effects of various kinds of saccharides and their concentrations on the viability of freeze-dried platelets were investigated.Raising the concentration of trehalose to 20%,the recovery rate of nonactived platelets can reach to 85.7%.For various kinds of saccharides with the same concentration of 20%,the recovery rate of nonactived platelets is above 70%,trehalose is slightly higher than others,however there is no statistically significant difference among them.

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周新丽,刘宝林,张绍志,陈光明,祝宏,朱发明.糖的浓度和种类对人血小板冷冻干燥保存的影响[J].上海理工大学学报,2007,(6):534-538.

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  • 最后修改日期:2007-07-03
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