龙井茶茶树种质资源低温保存特性研究
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国家自然科学基金资助项目(3171101125)


Study on Cryopreservation Characteristics of Longjing Tea Seeds Resources
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    摘要:

    针对茶树种子在国际种质资源库建设标准下(干燥至3%~7%的含水量,-20 ℃保存)长期保存的可能性,研究了龙井茶种子的脱水敏感性及低温保存特性。结果表明:龙井茶不是顽拗性种子,当含水量在7%~9%时,其生命力最为旺盛;在-20 ℃环境下保存超过1星期后,其活性显著降低,超过3个月后种子全部失活。通过冷冻干燥技术和扫描电镜,观察了茶种子内部胚轴、胚乳和断面形态,发现当保存温度维持在-20 ℃时,种子失活的原因很可能是内部遭受了冰晶破坏性的损伤。

    Abstract:

    To gain more insights into the possibility of conservation under international seeds bank conditions (3%~7% moisture content, at -20℃), the desiccation sensitivity and cryopreservation characteristic of the tea (Camellia sinensis) seeds were investigated. The results show that tea seeds are not recalcitrant, as they can tolerate drying to 7%~9% moisture content without significant viability loss. However, survival of two months cold-stratified seeds dry to 7%~9% declined sharply after one week storage at -20℃, and no seeds survive after three months. Detailed studies using scanning electron microscope of the cross sections revealed that when storage temperature was maintained at -20℃, there was a fatal cryoinjury in both axis and cotyledon, occurred mainly as a result of freezable water becoming ice.

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宋丹萍,JAGANATHANGaneshK.,刘威,刘宝林.龙井茶茶树种质资源低温保存特性研究[J].上海理工大学学报,2018,40(5):481-488.

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  • 收稿日期:2017-12-21
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  • 在线发布日期: 2018-12-20
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