The influence of voltage gradient and permeable reaction barrier (PRB) material on the remediation of Cd-contaminated soil was studied by immobilized microorganism technology to prepare fly ash-yeast pellets, and combined with electrokinetic-permeable reaction wall (EK-PRB) technology. The repair period was set at 120 h; the initial water content was 30%; the electrolyte was CA and EDTA. Six sets of voltage gradients (1.0, 2.0, 3.0, 4.0, 5.0, 2.5 V/cm) were set, and the most suitable voltage gradient was obtained. The effect of PRB materials (fly ash-yeast pellets, fly ash) were compared. A total of seven sets of tests were conducted. The results showed that the increase of voltage gradient in the range of 1.0～5.0 V/cm has a facilitating effect on Cd removal, and the removal rate of Cd can be increased by 23.4%. Fly ash-yeast pellets are more effective as PRB materials for the remediation of Cd-contaminated soil. Considering the cost of electrical energy consumption and application, the voltage gradient is selected as 2.5 V/cm and the PRB material is fly ash-yeast pellets. The removal rate of Cd can reach 53.7% , which reflects the feasibility and remediation advantages of immobilized microorganisms combined with EK-PRB technology, and provides basic support for the subsequent in-depth study of the removal mechanism and material innovation.