Industrial symbiosis chain management is an effective means of improving economic efficiency and environmental sustainability between different chains. A two-level industrial symbiosis chain model consisting of a single supplier and a single manufacturer was developed. The Stackelberg game was used to analyze the optimal pricing and profit model for each participant under different rights dominant models of suppliers and manufacturers. The impact of the degree of symbiosis, waste utilization and unit profit value on the revenue of the industrial symbiosis chain was also analyzed. Results show that different rights dominance leads to different optimal prices and maximum profits for each player. Suppliers and manufacturers are in the lead in price and revenue decisions regardless of who dominates. The total revenue of the industrial symbiosis chain is higher when the manufacturer is dominant than when the supplier is dominant. As the degree of symbiosis and waste utilization increases, the symbiotic firms can earn more profits. The validity of the conclusions is finally verified by the analysis of arithmetic examples.