Abstract:Based on the biological study of typical enteric viruses, norovirus, adenovirus, and rotavirus were selected as the focus of this research. The study was conducted at a sludge treatment facility in Anhui Province, where the treatment processes employed include centrifugal concentration, hydrolysis, anaerobic digestion, mechanical dewatering, and solar drying. The primary objective of this research is to evaluate the effectiveness of different sludge treatment processes in the removal of these viruses. This study utilized real-time fluorescence quantitative PCR technology to quantitatively analyze the virus content in various stages of sludge treatment. Research shows that anaerobic digestion of sludge is the unit with the highest removal efficiency for norovirus and adenovirus, with logarithmic removal rates of 0.68 lg and 0.61 lg, respectively. The solar drying unit is the unit with the highest removal efficiency for rotavirus, with logarithmic removal rate of 0.73 lg. The total amount of Norovirus, adenovirus and rotavirus removed by this sludge treatment process reached 98.7%, 97.6% and 98.4%. However, due to the high total amount of virus in the sludge, the final content of sludge into the environment was 1.52 × 104 copies/g DW, 5.17 × 104 copies/g DW, 7.92 × 103 copies/g DW.